淡江大學 | 中文 | English

 

陳銘凱（Ming-Kai Chern）

酵素學、蛋白質工程、蛋白質體學、基因工程、酵母菌細胞功能與遺傳

淡江大學化學系 專任教師 (2009.8~迄今)
淡江大學生命科學研究所 專任教師 (2003.8~2009.7)
中央研究院分生所博士後研究員 (1998.9~2003.7)
美國羅格斯大學 博士 (1998)
臺北醫學院牙醫學學士 (1990)

陳銘凱

教師歷程系統

聯絡電話：(02) 2621-5656 ext.3039

研究室：C - 107

實驗室：C - 118 ~ 119

Email: mkchern@mail.tku.edu.tw

研究專長及興趣

酵素學、蛋白質工程、蛋白質體學、基因工程、 酵母菌細胞功能與遺傳

代表性著作

I. 期刊論文(Referred papers)

  1. Chern, M.-K. & Pietruszko, R., 1998 (Sep.1); Human aldehyde dehydrogenase E3 isozyme: the N-terminal primary structure. Biochem. J. 334: 487-488.
     
  2. Chern, M.-K. & Pietruszko, R., 1999 (Jun.); Evidence for mitochondrial localization of betaine aldehyde dehydrogenase in rat liver-purification,characterization and comparison with human cytoplasmic E3 isozyme. Biochemistry and Cell Biology 77: 179-187.
     
  3. Ambroziak, W., Izaguirre, G., Abriola, D., Chern, M.-K., Pietruszko, R. 1999; Metabolism of retinaldehyde by human liver and kidney. Adv. Exp. Med. Biol. 463: 205-211.
     
  4. Chern, M.-K., Wu, T.-C., Hsieh, C.-H., Chou, C.-C., Liu, L.-F., Kuan, I.-C., Yeh, Y.-H., Hsiao, C.-D., Tam, M.F. 2000 (Jul. 28); Tyr115, Gln165 and Trp209 contribute to the 1, 2-epoxy-3- (p-nitrophenoxy)propane-conjugation activity of glutathione S-transferase cGSTM1-1. J. Mol. Biol. 300: 1257-1269.
     
  5. Chern, M.-K., Gage, D.A., Pietruszko, R. 2000 (Dec.); Betaine aldehyde, betaine, and choline levels in rat liver during ethanol metabolism. Biochem. Pharmacol. 60: 1629-1637.
     
  6. Pietruszko, R. and Chern, M.-K. 2001 (Jan.); Betaine aldehyde dehydrogenase from rat liver mitochondrial matrix. Chemico-biological interactions 130-132(1-3): 193-9.
     
  7. Chern MK, Chang KN, Liu LF, Tam TC, Liu YC, Liang YL, Tam MF. 2002 (May); Yeast ribosomal protein L12 is a substrate of protein arginine methyltransferase (RMT2). Journal of Biological Chemistry 277: 15345-15353.
     
  8. Wang, San-Lang, Chen,Yen-Hsu, Wang, Chuan-Lu, Yen, Yue-Horng, Chern, Ming-Kai. 2005 (Apr.); Purification and characterization of a serine protease extracellularly produced by Aspergillus fumigatus in a shrimp and crab shell powder medium Enzyme and Microbial Technology 36 : 660-665.
     
  9. Wang SL, Kao TY, Wang CL, Yen YH, Chern MK, Chen YH. 2006 (Aug.); A solvent stable metalloprotease produced by Bacillus sp. TKU004 and its application in the deproteinization of squid pen for beta-chitin preparation. Enzyme and Microbial Technology 39 :724-731.
     
  10. Tseng SF, Huang TW, Chen CW, Chern MK, Tam MF, Teng SC. 2006 (May); ShyA, a membrane protein for proper septation of hyphae in Streptomyces. Biochem Biophys Res Commun. 343:369-77.
     
  11. Chen YH , Chen WL, Wang YH, Huang MY and Chern MK. 2007 (Apr.); Spatiotemporal expression of zebrafish D- amino acid oxidase during early embryogenesis. Fish Physiol. Biochem. 33 : 73-80.
     
  12. Shih-Juei Wang, Chi-yang Yu, Cheng-Kang Lee, Ming-Kai Chern and I-Ching Kuan. 2008 (Aug.); Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to hydrogen peroxide. Biotechnology letters 30 : 1415-1422.
     
  13. Ming-Kai Chern*, Wei-Jyh Shiah, Jyun-Jie Chen , Tzung-You Tsai , Hsin-Yin Lin, Chien-Wei Liu (2009), Single-step protein purification by back flush in ion exchange chromatography, Analytical Biochemistry , 392: 174–176. 
     
  14. Hsin-Ying Lin, Sey-En Lin, Su-Fang Chien, Ming-Kai Chern* (2011), Electroporation for three commonly used yeast strains for two-hybrid screening experiments, Analytical Biochemistry, 416: 117-119, Epub 29 April 2011

  15. Ming-Kai Chern*,  Huang-Yi Li, Po-Fan Chen,Su-Fang Chien* (2012), Taro α-galactosidase: A new gene product for blood conversion, Biocatalysis and Agricultural Biotechnology, 1: 135-139.

  16. Feng-ing Wang, Ming-kai Chern, Chi-wang Li, Min Yan, and Yu-hsien Hsieh (2012), Prevalence and antibiotic resistance of Listeria species in food products in Taipei, Taiwan, The African Journal of Microbiology Research, 6: 4702-4706.
     
  17. Jen-Hong Yen, Sheng-Fu Chen, Ming-Kai Chern, Po-Chien Lu* (2014), The effect of turbulent viscous shear stress on red blood cell hemolysis, J. Artif. Organs, 17:178-185.

  18. Jen-Hong Yen, Sheng-Fu Chen, Ming-Kai Chern, Po-Chien Lu* (2015), The effects of extensional stress on red blood cell hemolysis, Biomedical Engineering: Applications, Basis and Communications 27: 1550042 (11 pages)

 

II. 研討會論文(Conference papers) 

  1.  Wu, T.-C., Chern, M.-K., Hsieh, C.-H., Chou, C.-C., Liu, L.-F., Kuan, I.-C., Yeh, Y.-H., Hsiao,C.-D., Tam, M.F. 2000 (May); Struture of functional study of glutathione S-transferase cGSTM1-1 complexed with S-glutathione conjugated 1,2-epoxy-3-(p-nitrophenoxy) propane. 第六屆生物物理新知研討會, No.S22, p.42, 國立清華大學.
  2. Min-Kai Chern, Gin-Wen Cheng, Wen-Teish Chang, San-Lang Wang. 2004 (Oct.); Production of proteases from shellfish chitin wastes by Bacillus cereus Asia Pacific Confederation of Chemical Engineering (APCChE2004) , Kitakyushu , Japan.
  3. Ming-Chun Chiu, Tzung-You Tsai, Duan-Yue Hung, Ming-Kai Chern; Cloning, Homologous Expression, and Purification of ADH3 from Mitochondrial Matrix of the Yeast Saccahromyces cerivisiae. 2005 Annual Meeting of Chemical Society Located in Taipei; BC0400. 2005 (Nov.)
  4. Yi-Chih Tzeng, Ching-Yuan Mao, Ming-Kai Chern; Cloning, Expression, and Purification of Ald2p and Ald3p of the Yeast Saccharomyces cerevisiae. 2005 Annual Meeting of Chemical Society Located in Taipei; BC0401. 2005 (Nov.)
  5. Shu-Ping Wen, Kuo-Chen Chen, Hsin-Ying Lin, Tzung-You Tsai, I-Te Chen, Chih-Hsiung Cheng, Ming-Kai Chern; Yeast Two-hybrid Screening of YJR129C, a Putative Protein Methyltransferase Gene of the Yeast Saccharomyces cerevisiae. 2005 Annual Meeting of Chemical Society Located in Taipei; BC0403. 2005 (Nov.)
  6. Tzung-You Tsai, Ming-Kai Chern; Purification and Characterization of ALD4p from the Yeast Saccahromyces cerivisiae. 2006 Annual Meeting of Chemical Society Located in Taipei; B-057. 2006 (Nov.)
  7. Hsin-Ying Lin, Sey-En Lin, Ming-Kai Chern; Yeast Two-hybrid Screening of EBNA2 Interacting Proteins in the Human Lymphocyte cDNA Library. 臺北醫學大學95學年度師生聯合學術研究發表會；A-18. 2007 (May)
  8. Wei-Jyh Shiah , Yu-Chun Chen , Ming-Kai Chern. 2008 (Jul.); Non-degraded expression and one-step purification of yeast ALD6 enzyme in E. coli . The First International Symposium on Process Chemistry [ISPC 08] , Kyoto, Japan.
  9. Ming-Kai Chern, Yung-Yu Yang , How-Jing Lee; Characterization of a novel endonuclease of Drosophila retrotransposon and its application to the mosquito control. The 3rd International Symposium on Biological Control of Arthropods, Christchurch, ew Zealand; Session 4.2. 2009 (Feb.)
  10. Ming-Kai Chern (2010, Jun). An unexpected way out - protein purification by back flush in conventional chromatography. BioJohor 2010-The Second International Biotechnology and Biodiversity Conference, Johor Bahru, Malasia.
  11. Ming-Kai Chern (2010, Nov). A solvent stable metalloprotease produced by Bacillus sp. TKU004 and its application in the deproteinization of squid pen for beta-chitin preparation. The 2nd International and Cross-Strait Chitin and Chitosan Symposium , Qianjiang, Hubei, China.
  12.  Hsin-Ying Lin and Ming-Kai Chern, A general protocol of improved transformation by electroporation for yeast two-hybrid screening experiment, 2011 Annual Yeast Meeting in Taiwan. Yang-Ming University, Taipei. 2011 (Sep.)
  13. Ming-Kai Chern; A One-plus-one Dimensional Gel Analysis for Identification of Protein Methylation in Yeast. The International Symposium on Science Research VNU University of Science 27-28 April 2015, Le Van Third Grand Lecture Hall, 19-Le Thanh Tong Str., Hoan Kiem Dist., Hanoi-Vietnam
  14. Ming-Kai Chern; Probability amplitude: from Feynman rules to the reciprocal relationship and the Born rule. Joint Symposium of Asia Five Universities, The 6th OPU-KIST-ECUST-TKU Joint Symposium & The 5th OPU-TKU Joint Symposium & The 3rd OPU-FZU Joint Symposium, November 9 (Thu) - 11 (Sat), 2017, Osaka Prefecture University, Sakai, Osaka, Japan
  15.  Ming-Kai Chern; Screening of high efficiency fermenting yeast strains, The 7th ECUST-TKU-KIST-OPU on Advanced Materials and Their Applications November 1~3, 2018; East China University of Science and Technology, Yifu Building, Lecture Hall, Xuhui Campus, 130 Meilong Road, Xihui District, Shanghai, China
  16. Ming-Kai Chern; Cellular and Molecular Biology Laboratory, March 30, 2019, Kyoto/Osaka-Taiwan International BNCT Conference at Osaka Medical College, Osaka, Japan
  17. Ming-Kai Chern; Yeast Two-hybrid Screening of EBNA2 Interacting Proteins in the Human Lymphocyte cDNA Library, The 7th HCMUT-TKU-OPUKMITL-DLU-TNU Joint Symposium on Chemistry, Environment, Natural Sciences and Technologies October 25, 2019 Ho Chi Minh City, Vietnam

III. 專利(Patent)

  1. 蛋白質純化方法 (Method for Purifying Protein)：中華民國/第I 400128號, Jul. 2013：美國/第8354511號, Oct. 2012
  2. 水溶性卵殼膜粉末之製備方法；中華民國專利 (I631951，2018通過)
  3. 微針元件的製造方法與微針模具的製造方法：中華民國(發明第I698263號)和日本(特許第6731024号)專利 (2020 Jul. 通過)

IV. 近期參與研究計畫：

  1. 2016/11/01 勤億蛋品科技股份有限公司 - 水溶性卵殼膜之製備條件與量產研究計畫 (主持人)
  2. 2018/09/01 孟鄉生化科技股份有限公司 - 無網布生物可分解水凝膜及水溶性生物可分解奈米纖維抗菌敷材 (主持人)
  3. 2019/08/01 欣梗科技股份有限公司 - 產品測試計畫 (主持人)
  4. 2020/06/01 產學合作計畫(孟鄉生化科技股份有限公司) -客製化美容微針製程 (共同主持人)